____________ is used to determine if an open globe injury exists by applying fluorescein to the injury site.

• A. Seidel's test
• B. Slit lamp examination
• C. Rose bengal staining
• D. Lissamine green staining

Answer: (A)

Open globe injuries are checked for loss of globe integrity by using fluorescein to look for leakage from the wound. The Seidel test applies fluorescein to the injury and, under cobalt blue light, looks for a dilute stream of aqueous humor that dilutes the dye. If such a clear stream appears within the fluorescein-stained area, that Seidel phenomenon confirms a breach in the globe. A negative result doesn’t rule out injury entirely, but a positive finding is a clear sign of leakage and a surgical emergency. Slit lamp examination is a general visualization technique, not specifically about detecting leakage with fluorescein. Rose bengal and Lissamine green staining are used to assess surface cells and contact lens-related issues, not to reveal globe integrity through leakage.